GeneCAPSULE™ is an electro-elution tool for rapid recovery of PCR products, DNA fragments and proteins from agarose and polyacrylamide gels. GeneCAPSULE™ consists of mainly two parts, Gel-PICK™ – for picking DNA or protein band from the gel, and the Gene-TRAP™, which traps the migrating DNA or protein during electroelution. After excising DNA or protein band from the gel with Gel- PICK™, the GeneCAPSULE™ assembly is then placed in a gel box with current applied. Under the influence of an electrical field, DNA and protein molecules migrate toward the positive (+) terminal of the electrophoresis apparatus and captured by Gene-TRAP™ and become bound to the membrane (See Fig 1).
Electroelution through GeneCAPSULE™ is a gentle method that eliminates the risks of damage to DNA or protein samples commonly encountered in use of glass milk, spin columns, binding columns and other popular techniques. The GeneCAPSULE™ method is simple to perform and eliminates additional steps involving washing, spinning, heating or precipitation etc. The recovered DNA is of highest quality for use in molecular manipulations such as ligation, restriction enzyme digestion, sequencing, amplification, random priming, and other enzymatic reactions.
GeneCAPSULE™Accessory Kit (Cat. # 786-004)– Supplied separately; It prevents GeneCAPSULE™ from floating as shown in Fig. 1. The accessory kit consists of one GeneCAPSULE™-Weight and plastic Forceps. The Weight is designed to trap GeneCAPSULE™ when submerged under buffer and prevents it from floating or changing direction during electroelution. Forceps can be used for manipulation of GeneCAPSULE™ in and out of buffer tank.